CONSTRUCTION AND APPLICATION OF HIGHLY SELECTIVE SENSORS AND BIOSENSORS USING NON-CONDUCTING ELECTROPOLYMERIZED FILMS

A. Curulli¹, and G. Palleschi²

¹Centro CNR di studio per L'Elettrochimica e la Chimica Fisica delle Interfasi Via del CastroLaurenziano 7 000161 Roma

²Dipartimento di Scienze e Tecnologie Chimiche Università di Roma Tor Vergata Via della Ricerca Scientifica 00133 Roma.

Different kind of macrosensors have been assembled using several non-conducting electropolymerized films. Different techniques of film electropolymerization such as cyclic voltammetry and chronoamperometry are compared. Film function, including the prevention of interferences and fouling problems and immobilization of the enzymes are described and optimised. We have immobilized oxidase [1] and dehydrogenase enzymes [2] onto electropolymerized films with different techniques, depending in the nature of the enzyme. We have, also, developed an interference-free highly selective sensor for the detection of NO in biological media [3], using non-conducting electrosynthesized films

Keywords: electropolymerization, sensors, biosensors.